Coated pits act as molecular filters.
نویسندگان
چکیده
منابع مشابه
Potassium-dependent assembly of coated pits: new coated pits form as planar clathrin lattices
Previous studies have shown that when human fibroblasts are depleted of intracellular K+, coated pits disappear from the cell surface and the receptor-mediated endocytosis of low density lipoprotein (LDL) is inhibited. We have now used the K+ depletion protocol to study several aspects of coated pit function. First, since coated pits rapidly form when K+-depleted fibroblasts are incubated in th...
متن کاملUnderstanding living clathrin-coated pits.
Most knowledge of clathrin-mediated endocytosis has been gained by biochemical fractionation and in vitro assays. Recently, the study of endocytosis has extended into the living cell. The tracking of individual clathrin-coated pits and vesicles (CCPs and CCVs) has provided new insight into understanding the dynamic nature of CCPs. The use of total internal reflection fluorescence microscopy (TI...
متن کاملDistinct Dynamics of Endocytic Clathrin-Coated Pits and Coated Plaques
Clathrin is the scaffold of a conserved molecular machinery that has evolved to capture membrane patches, which then pinch off to become traffic carriers. These carriers are the principal vehicles of receptor-mediated endocytosis and are the major route of traffic from plasma membrane to endosomes. We report here the use of in vivo imaging data, obtained from spinning disk confocal and total in...
متن کاملCoated pits bring in the yolk
n the early 1960s, while analyzing electron micrographs of mosquito oocytes in search of hints about chromosomal structure, Thomas Roth's roving eye became more interested in the cell surface, which was covered with pit structures lined by a cytoplasmic coat of " bristles. " When he showed the find to his I The schematic model figure in the paper has remarkably withstood the test of time even a...
متن کاملEps15 is a component of clathrin-coated pits and vesicles and is located at the rim of coated pits.
Eps15, a phosphorylation substrate of the epidermal growth factor (EGF) receptor kinase, has been shown to bind to the alpha-subunit of the clathrin-associated protein complex AP-2. Here we report that in cells, virtually all Eps15 interacts with the cytosol and membrane-bound forms of AP-2. This association is not affected by the treatment of cells with EGF. Immunofluorescence microscopy revea...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1980
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.77.7.4156